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Calcium carbonate supplementation suppressed aging-induced high gut permeability (A) Flow chart for testing the intestinal effects of calcium carbonate supplementation in senile osteoporotic mice. (B) Representative images of H&E staining of colonic tissue and intestinal morphology studies measure cumulative scores, including inflammation infiltration, crypt damage, and goblet cell damage. (C) Representative images of AB-PAS staining of colonic tissue and quantitative analysis. (D) Representative images of immunohistochemistry staining of colonic tissue and quantitative analysis. (E) The mRNA expression level of Ocln , Glp-2r , Zo-1 , <t>and</t> <t>Claudin-1</t> . (F) The concentration of the inflammatory cytokines TNF-α, IL-17A, and anti-inflammatory cytokines IL-10 and IL-4 in serum. n = 5/all group, n = number of mice per group (biological replicates). n = 5/all group, n represents the number of mice per group (biological replicates). The values are mean ± SD of at least 3 independent experiments, n.s. p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
Anti Claudin 1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti claudin 1 antibody/product/Proteintech
Average 96 stars, based on 1 article reviews
anti claudin 1 antibody - by Bioz Stars, 2026-03
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claudin 1 antibody  (Proteintech)
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Proteintech claudin 1 antibody
Calcium carbonate supplementation suppressed aging-induced high gut permeability (A) Flow chart for testing the intestinal effects of calcium carbonate supplementation in senile osteoporotic mice. (B) Representative images of H&E staining of colonic tissue and intestinal morphology studies measure cumulative scores, including inflammation infiltration, crypt damage, and goblet cell damage. (C) Representative images of AB-PAS staining of colonic tissue and quantitative analysis. (D) Representative images of immunohistochemistry staining of colonic tissue and quantitative analysis. (E) The mRNA expression level of Ocln , Glp-2r , Zo-1 , <t>and</t> <t>Claudin-1</t> . (F) The concentration of the inflammatory cytokines TNF-α, IL-17A, and anti-inflammatory cytokines IL-10 and IL-4 in serum. n = 5/all group, n = number of mice per group (biological replicates). n = 5/all group, n represents the number of mice per group (biological replicates). The values are mean ± SD of at least 3 independent experiments, n.s. p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
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Proteintech claudin 1
Calcium carbonate supplementation suppressed aging-induced high gut permeability (A) Flow chart for testing the intestinal effects of calcium carbonate supplementation in senile osteoporotic mice. (B) Representative images of H&E staining of colonic tissue and intestinal morphology studies measure cumulative scores, including inflammation infiltration, crypt damage, and goblet cell damage. (C) Representative images of AB-PAS staining of colonic tissue and quantitative analysis. (D) Representative images of immunohistochemistry staining of colonic tissue and quantitative analysis. (E) The mRNA expression level of Ocln , Glp-2r , Zo-1 , <t>and</t> <t>Claudin-1</t> . (F) The concentration of the inflammatory cytokines TNF-α, IL-17A, and anti-inflammatory cytokines IL-10 and IL-4 in serum. n = 5/all group, n = number of mice per group (biological replicates). n = 5/all group, n represents the number of mice per group (biological replicates). The values are mean ± SD of at least 3 independent experiments, n.s. p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
Claudin 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/claudin 1/product/Proteintech
Average 96 stars, based on 1 article reviews
claudin 1 - by Bioz Stars, 2026-03
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claudin1  (Proteintech)
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Calcium carbonate supplementation suppressed aging-induced high gut permeability (A) Flow chart for testing the intestinal effects of calcium carbonate supplementation in senile osteoporotic mice. (B) Representative images of H&E staining of colonic tissue and intestinal morphology studies measure cumulative scores, including inflammation infiltration, crypt damage, and goblet cell damage. (C) Representative images of AB-PAS staining of colonic tissue and quantitative analysis. (D) Representative images of immunohistochemistry staining of colonic tissue and quantitative analysis. (E) The mRNA expression level of Ocln , Glp-2r , Zo-1 , <t>and</t> <t>Claudin-1</t> . (F) The concentration of the inflammatory cytokines TNF-α, IL-17A, and anti-inflammatory cytokines IL-10 and IL-4 in serum. n = 5/all group, n = number of mice per group (biological replicates). n = 5/all group, n represents the number of mice per group (biological replicates). The values are mean ± SD of at least 3 independent experiments, n.s. p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
Claudin1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/claudin1/product/Proteintech
Average 96 stars, based on 1 article reviews
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Calcium carbonate supplementation suppressed aging-induced high gut permeability (A) Flow chart for testing the intestinal effects of calcium carbonate supplementation in senile osteoporotic mice. (B) Representative images of H&E staining of colonic tissue and intestinal morphology studies measure cumulative scores, including inflammation infiltration, crypt damage, and goblet cell damage. (C) Representative images of AB-PAS staining of colonic tissue and quantitative analysis. (D) Representative images of immunohistochemistry staining of colonic tissue and quantitative analysis. (E) The mRNA expression level of Ocln , Glp-2r , Zo-1 , and Claudin-1 . (F) The concentration of the inflammatory cytokines TNF-α, IL-17A, and anti-inflammatory cytokines IL-10 and IL-4 in serum. n = 5/all group, n = number of mice per group (biological replicates). n = 5/all group, n represents the number of mice per group (biological replicates). The values are mean ± SD of at least 3 independent experiments, n.s. p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Journal: iScience

Article Title: Calcium carbonate supplementation exacerbated gut microenvironment disruption in senile osteoporosis mice

doi: 10.1016/j.isci.2025.114530

Figure Lengend Snippet: Calcium carbonate supplementation suppressed aging-induced high gut permeability (A) Flow chart for testing the intestinal effects of calcium carbonate supplementation in senile osteoporotic mice. (B) Representative images of H&E staining of colonic tissue and intestinal morphology studies measure cumulative scores, including inflammation infiltration, crypt damage, and goblet cell damage. (C) Representative images of AB-PAS staining of colonic tissue and quantitative analysis. (D) Representative images of immunohistochemistry staining of colonic tissue and quantitative analysis. (E) The mRNA expression level of Ocln , Glp-2r , Zo-1 , and Claudin-1 . (F) The concentration of the inflammatory cytokines TNF-α, IL-17A, and anti-inflammatory cytokines IL-10 and IL-4 in serum. n = 5/all group, n = number of mice per group (biological replicates). n = 5/all group, n represents the number of mice per group (biological replicates). The values are mean ± SD of at least 3 independent experiments, n.s. p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: For immunohistochemistry staining, after antigen extraction, each section was incubated with anti-ZO-1 antibody (#21773, 1:200, Proteintech, China), anti-Claudin-1 antibody (#13050, 1:200, Proteintech, China), and anti-Occludin antibody (#27260, 1: 200, Proteintech, China) at 4°C overnight.

Techniques: Calcium Carbonate, Permeability, Staining, Immunohistochemistry, Expressing, Concentration Assay

Calcium carbonate supplementation suppressed aging-induced high gut permeability (A) Flow chart for testing the intestinal effects of calcium carbonate supplementation in senile osteoporotic mice. (B) Representative images of H&E staining of colonic tissue and intestinal morphology studies measure cumulative scores, including inflammation infiltration, crypt damage, and goblet cell damage. (C) Representative images of AB-PAS staining of colonic tissue and quantitative analysis. (D) Representative images of immunohistochemistry staining of colonic tissue and quantitative analysis. (E) The mRNA expression level of Ocln , Glp-2r , Zo-1 , and Claudin-1 . (F) The concentration of the inflammatory cytokines TNF-α, IL-17A, and anti-inflammatory cytokines IL-10 and IL-4 in serum. n = 5/all group, n = number of mice per group (biological replicates). n = 5/all group, n represents the number of mice per group (biological replicates). The values are mean ± SD of at least 3 independent experiments, n.s. p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Journal: iScience

Article Title: Calcium carbonate supplementation exacerbated gut microenvironment disruption in senile osteoporosis mice

doi: 10.1016/j.isci.2025.114530

Figure Lengend Snippet: Calcium carbonate supplementation suppressed aging-induced high gut permeability (A) Flow chart for testing the intestinal effects of calcium carbonate supplementation in senile osteoporotic mice. (B) Representative images of H&E staining of colonic tissue and intestinal morphology studies measure cumulative scores, including inflammation infiltration, crypt damage, and goblet cell damage. (C) Representative images of AB-PAS staining of colonic tissue and quantitative analysis. (D) Representative images of immunohistochemistry staining of colonic tissue and quantitative analysis. (E) The mRNA expression level of Ocln , Glp-2r , Zo-1 , and Claudin-1 . (F) The concentration of the inflammatory cytokines TNF-α, IL-17A, and anti-inflammatory cytokines IL-10 and IL-4 in serum. n = 5/all group, n = number of mice per group (biological replicates). n = 5/all group, n represents the number of mice per group (biological replicates). The values are mean ± SD of at least 3 independent experiments, n.s. p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Claudin-1 Antibody , Proteintech , Cat# 13050-1-AP; RRID: AB_2079881.

Techniques: Calcium Carbonate, Permeability, Staining, Immunohistochemistry, Expressing, Concentration Assay